micro Affinity Chromatography
Separation of mouse IgG on Protein A from bovine
serum albumin on Sepharose microcolumn.
3.4.8.
Sample: IgG 2μg/μL, BSA 4.6μg/μL. Injected volume 75 μL. Mobile phase PBS (0.14MNaCl, 3.8mM KCl, EDTA 1mM, phosphate 18mM, pH=7.4. Eluant 1M HCl, volume 5μL. Sample on column flow rate 5mL/sec, elution flow rate 2μL/sec. Column capacity 20mg/mL, column volume 10μL. Flow cell volume 6μL, optical path 3mm.
